A novel 'Gold on Gold' biosensing scheme for an on-fiber immunoassay

N. Punjabi; Jitendra Satija; S. Mukherji

doi:10.1117/12.2081233

5 May 2015 A novel 'Gold on Gold' biosensing scheme for an on-fiber immunoassay

N. Punjabi, Jitendra Satija, S. Mukherji

Proceedings Volume 9506, Optical Sensors 2015; 95062H (2015) https://doi.org/10.1117/12.2081233
Event: SPIE Optics + Optoelectronics, 2015, Prague, Czech Republic

Abstract

In this paper, we propose a novel „gold on gold‟ biosensing scheme for absorbance based fiber-optic biosensor. First, a self-assembled monolayer of gold nanoparticles is formed at the sensing region of the fiber-optic probe by incubating an amino-silanized probe in a colloidal gold solution. Thereafter, the receptor moieties, i.e. Human immunoglobulin G (HIgG) were immobilized by using standard alkanethiol and classic carbodiimide coupling chemistry. Finally, biosensing experiments were performed with different concentrations of gold nanoparticle-tagged analyte, i.e. Goat anti- Human immunoglobulin G (Nanogold-GaHIgG). The sensor response was observed to be more than five-fold compared to the control bioassay, in which the sensor matrix was devoid of gold nanoparticle film. Also, the response was found to be ~10 times higher compared to the FITC-tagged scheme and ~14.5 times better compared to untagged scheme. This novel scheme also demonstrated the potential in improving the limit of detection for the fiber-optic biosensors.

Citation Download Citation

N. Punjabi, Jitendra Satija, and S. Mukherji "A novel 'Gold on Gold' biosensing scheme for an on-fiber immunoassay", Proc. SPIE 9506, Optical Sensors 2015, 95062H (5 May 2015); https://doi.org/10.1117/12.2081233

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