Analysis of antifreeze protein activity using colorimetric gold nanosensors

Xu Jing; Ho-seok Choi; Ji-In Park; Young-Pil Kim

doi:10.1117/12.2189039

8 July 2015 Analysis of antifreeze protein activity using colorimetric gold nanosensors

Xu Jing, Ho-seok Choi, Ji-In Park, Young-Pil Kim

Proceedings Volume 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015; 95230C (2015) https://doi.org/10.1117/12.2189039
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 2015, Jeju, Korea, Republic of

Abstract

High activity and long stability of antifreeze proteins (AFPs), also known as ice-binding proteins (IBPs), are necessary for exerting their physiological functions in biotechnology and cryomedicine. Here we report a simple analysis of antifreeze protein activity and stability based on self-assembly of gold nanoparticles (AuNPs) via freezing and thawing cycles. While the mercaptosuccinic acid-capped AuNP (MSA-AuNP) was easily self-assembled after a freezing/thawing cycle, due to the mechanical attack of ice crystal on the MSA-AuNP surface, the presence of AFP impeded the self-assembly of MSA-AuNP via the interaction of AFP with ice crystals via freezing and thawing cycles, which led to a strong color in the MSA-AuNP solution. As a result, the aggregation parameter (E₅₂₀/E₆₅₀) of MSA-AuNP showed the rapid detection of both activity and stability of AFPs. We suggest that our newly developed method is very suitable for measuring antifreeze activity and stability in a simple and rapid manner with reliable quantification.

Citation Download Citation

Xu Jing, Ho-seok Choi, Ji-In Park, and Young-Pil Kim "Analysis of antifreeze protein activity using colorimetric gold nanosensors", Proc. SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 95230C (8 July 2015); https://doi.org/10.1117/12.2189039

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available