Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA

Inhong Kim; Mijeong Kang; Han Young Woo; Jin-Woo Oh; Kwangseuk Kyhm

doi:10.1117/12.2190332

8 July 2015 Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA

Inhong Kim, Mijeong Kang, Han Young Woo, Jin-Woo Oh, Kwangseuk Kyhm

Proceedings Volume 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015; 95230N (2015) https://doi.org/10.1117/12.2190332
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 2015, Jeju, Korea, Republic of

Abstract

We have investigated that organic solvent (DMSO, dimethyl sulfoxide) modifies energy transfer efficiency between conjugated polymers (donors) and fluorescein-labeled single stranded DNAs (acceptors). In a mixture of buffer and organic solvent, fluorescence of the acceptors is significantly enhanced compared to that of pure water solution. This result can be attributed to change of the donor-acceptor environment such as decreased hydrophobicity of polymers, screening effect of organic solvent molecules, resulting in an enhanced energy transfer efficiency. Time-resolved fluorescence decay of the donors and the acceptors was modelled by considering the competition between the energy harvesting Foerster resonance energy transfer and the energy-wasting quenching. This enables to quantity that the Foerster distance (R₀ = 43.3 Å) and resonance energy transfer efficiency (E_FRET = 58.7 %) of pure buffer solution become R₀ = 38.6 Å and E_FRET = 48.0 % when 80% DMSO/buffer mixture is added.

Citation Download Citation

Inhong Kim, Mijeong Kang, Han Young Woo, Jin-Woo Oh, and Kwangseuk Kyhm "Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA", Proc. SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 95230N (8 July 2015); https://doi.org/10.1117/12.2190332

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