Image correlation based method for the analysis of collagen fibers patterns

Ramon G. T. Rosa; Sebastião Pratavieira; Cristina Kurachi

doi:10.1117/12.2180920

19 June 2015 Image correlation based method for the analysis of collagen fibers patterns

Ramon G. T. Rosa, Sebastião Pratavieira, Cristina Kurachi

Proceedings Volume 9531, Biophotonics South America; 95310B (2015) https://doi.org/10.1117/12.2180920
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil

Abstract

The collagen fibers are one of the most important structural proteins in skin, being responsible for its strength and flexibility. It is known that their properties, like fibers density, ordination and mean diameter can be affected by several skin conditions, what makes these properties a good parameter to be used on the diagnosis and evaluation of skin aging, cancer, healing, among other conditions. There is, however, a need for methods capable of analyzing quantitatively the organization patterns of these fibers. To address this need, we developed a method based on the autocorrelation function of the images that allows the construction of vector field plots of the fibers directions and does not require any kind of curve fitting or optimization. The analyzed images were obtained through Second Harmonic Generation Imaging Microscopy. This paper presents a concise review on the autocorrelation function and some of its applications to image processing, details the developed method and the results obtained through the analysis of hystopathological slides of landrace porcine skin. The method has high accuracy on the determination of the fibers direction and presents high performance. We look forward to perform further studies keeping track of different skin conditions over time.

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Ramon G. T. Rosa, Sebastião Pratavieira, and Cristina Kurachi "Image correlation based method for the analysis of collagen fibers patterns", Proc. SPIE 9531, Biophotonics South America, 95310B (19 June 2015); https://doi.org/10.1117/12.2180920

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KEYWORDS

Collagen

Skin

Second-harmonic generation

Image analysis

Microscopy

Anisotropy

Electroluminescent displays

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