Combined phosphorescence-holographic approach for singlet oxygen detection in biological media

I. V. Semenova; A. V. Belashov; D. M. Beltukova; N. V. Petrov; O. S. Vasyutinskii

doi:10.1117/12.2180909

19 June 2015 Combined phosphorescence-holographic approach for singlet oxygen detection in biological media

I. V. Semenova, A. V. Belashov, D. M. Beltukova, N. V. Petrov, O. S. Vasyutinskii

Proceedings Volume 9531, Biophotonics South America; 95310S (2015) https://doi.org/10.1117/12.2180909
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil

Abstract

The paper presents a novel combined approach aimed to detect and monitor singlet oxygen molecules in biological specimens by means of the simultaneous recording and monitoring of their deactivation dynamics in the two complementary channels: radiative and nonradiative. The approach involves both the direct registration of phosphorescence at the wavelength of about 1270 nm caused by radiative relaxation of excited singlet oxygen molecules and holographic recording of thermal disturbances in the medium produced by their nonradiative relaxation. The data provides a complete set of information on singlet oxygen location and dynamics in the medium. The approach was validated in the case study of photosensitized generation of singlet oxygen in onion cell structures.

Citation Download Citation

I. V. Semenova, A. V. Belashov, D. M. Beltukova, N. V. Petrov, and O. S. Vasyutinskii "Combined phosphorescence-holographic approach for singlet oxygen detection in biological media", Proc. SPIE 9531, Biophotonics South America, 95310S (19 June 2015); https://doi.org/10.1117/12.2180909

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