Methylene blue photodynamic therapy in rats' wound healing: 21 days follow-up

Vanda Sanderana Macêdo Carneiro; Maria Helena Chaves de Vasconcelos Catao; Rebeca Ferraz Menezes; Natália Costa Araújo; Marleny Elizabeth Martinez Gerbi

doi:10.1117/12.2181124

19 June 2015 Methylene blue photodynamic therapy in rats' wound healing: 21 days follow-up

Vanda Sanderana Macêdo Carneiro, Maria Helena Chaves de Vasconcelos Catao, Rebeca Ferraz Menezes, Natália Costa Araújo, Marleny Elizabeth Martinez Gerbi

Author Affiliations +

Proceedings Volume 9531, Biophotonics South America; 95311S (2015) https://doi.org/10.1117/12.2181124
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil

Abstract

The experimental evaluated the photodynamic therapy (PDT) in wound healing. It used 60 male rats, making two circular wounds at each animal. They were treated at 48hs intervals, with methylene blue (MB), low level laser treatment (LLLT) or both, thus resulting in PDT. The wounds were observed 01, 03, 07, 14 and 21 days after and then processed and subjected to HE staining to analyze granulation tissue, necrosis, epithelialization and collagen. After day 1, wounds treated with MB showed necrosis less intense than other groups, and the PDT group showed more intense granulation tissue. At day 3, reepithelialization was absent for half of injuries in the PDT group, and this group was also with lower collagen. However, at day 7, this same group presented reepithelialization more advanced than control group, which did not happen with those treated with MB or LLLT (p = 0.015). The results allow us to conclude that PDT difficulted reepithelization at 7th day and interfered in standard healing. However, when used separately, MB and LLLT interfered significantly compared to the control group, which did not happened to the PDT group. There was no significant difference between the treatment groups in other analysed times.

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Vanda Sanderana Macêdo Carneiro, Maria Helena Chaves de Vasconcelos Catao, Rebeca Ferraz Menezes, Natália Costa Araújo, and Marleny Elizabeth Martinez Gerbi "Methylene blue photodynamic therapy in rats' wound healing: 21 days follow-up", Proc. SPIE 9531, Biophotonics South America, 95311S (19 June 2015); https://doi.org/10.1117/12.2181124

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