Assembly and characterization of a fluorescence lifetime spectroscopy system for skin lesions diagnostic

Marcelo Saito Nogueira; Ramon Gabriel Teixeira Rosa; Sebastião Pratavieira; Camila de Paula D'Almeida; Cristina Kurachi

doi:10.1117/12.2180599

19 June 2015 Assembly and characterization of a fluorescence lifetime spectroscopy system for skin lesions diagnostic

Marcelo Saito Nogueira, Ramon Gabriel Teixeira Rosa, Sebastião Pratavieira, Camila de Paula D'Almeida, Cristina Kurachi

Proceedings Volume 9531, Biophotonics South America; 95313D (2015) https://doi.org/10.1117/12.2180599
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil

Abstract

The fluorescence spectra and fluorescence lifetime analysis in biological tissues has been presented as a technique of a great potential for tissue characterization for diagnostic purposes. The objective of this study is to assemble and characterize a fluorescence lifetime spectroscopy system for diagnostic of clinically similar skin lesions in vivo. The fluorescence lifetime measurements were performed using the Time Correlated Single Photon Counting (Becker & Hickl, Berlin, Germany) technique. Two lasers, one emitting at 378 nm and another at 445 nm, are used for excitation with 20, 50 and 80 MHz repetition rate. A bifurcated optical fiber probe conducts the excitation light to the sample, the collected light is transmitted through bandpass filters and delivered to a hybrid photomultiplier tube detector. The fluorescence spectra were obtained by using a portable spectrometer (Ocean Optics USB-2000-FLG) with the same excitation sources. An instrument response function of about 300 ps was obtained and the spectrum and fluorescence lifetime of a standard fluorescent molecule (Rhodamine 6G) was measured for the calibration of the system ((4.1 ± 0.3) ns). The assembled system was considered robust, well calibrated and will be used for clinical measurements of skin lesions.

Citation Download Citation

Marcelo Saito Nogueira, Ramon Gabriel Teixeira Rosa, Sebastião Pratavieira, Camila de Paula D'Almeida, and Cristina Kurachi "Assembly and characterization of a fluorescence lifetime spectroscopy system for skin lesions diagnostic", Proc. SPIE 9531, Biophotonics South America, 95313D (19 June 2015); https://doi.org/10.1117/12.2180599

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