Translator Disclaimer
19 June 2015 In situ visualization of dermal collagen dynamics during skin burn healing using second-harmonic-generation microscopy
Author Affiliations +
Proceedings Volume 9531, Biophotonics South America; 95313U (2015)
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil
Burn healing is a process to repair thermally damaged tissues. Although burn healing has many aspects, it is common for dynamics of collagen fiber, such as decomposition, production, or growth, to be closely related with burn healing. If such healing process can be visualized from the viewpoint of the collagen dynamics, one may obtain new findings regarding biological repairing mechanisms in the healing process. To this end, second-harmonic-generation (SHG) light will be an effective optical probe because of high selectivity and good image contrast to collagen molecules as well as high spatial resolution, optical three-dimensional (3D) sectioning, minimal invasiveness, deep penetration, the absence of interference from background light, and in situ measurement without additional staining. Furthermore, since SHG light arises from a non-centrosymmetric triple helix of three polypeptide chains in the collagen molecule, its intensity decreases and finally disappears when thermal denaturation caused by the skin burn changes the structure of this molecule to a centrosymmetric random coil. Therefore, optical assessment of skin burn has been investigated by SHG microscopy. In this paper, we applied SHG microscopy for in situ imaging of the healing process in animal skin burn and successfully visualized the decomposition, production, and growth of renewal collagen fibers as a series of time-lapse images in the same subject.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Takeshi Yasui, Eiji Hase, Ryosuke Tanaka, Shu-ichiro Fukushima, and Tsutomu Araki "In situ visualization of dermal collagen dynamics during skin burn healing using second-harmonic-generation microscopy", Proc. SPIE 9531, Biophotonics South America, 95313U (19 June 2015);

Back to Top