The optical unit in standard UM as well as two optimized designs for generating light sheet are reviewed and discussed. The optical characteristics of their light sheet, length and diameter of the line of focus and spatial intensity distribution along the light sheet, are compared. The experimental results demonstrate a marked improvement in reconstructions of representative biological specimens in the third design utilizing aspheric elements. Furthermore, we present our latest achievements in the clearing process of the specimens as well as detection signals by microscope’s objectives that improve further the image quality.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither SPIE nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the SPIE website.