Visualization of hemodynamics and light scattering in exposed brain of rat using multispectral image reconstruction based on Wiener estimation method

Izumi Nishidate; Tomohiro Ishizuka; Keiichiro Yoshida; Satoko Kawauchi; Shunichi Sato; Manabu Sato

doi:10.1117/12.2183759

14 July 2015 Visualization of hemodynamics and light scattering in exposed brain of rat using multispectral image reconstruction based on Wiener estimation method

Izumi Nishidate, Tomohiro Ishizuka, Keiichiro Yoshida, Satoko Kawauchi, Shunichi Sato, Manabu Sato

Author Affiliations +

Proceedings Volume 9536, Advanced Microscopy Techniques IV; and Neurophotonics II; 95360Z (2015) https://doi.org/10.1117/12.2183759
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

We investigate a method to estimate the spectral images of reduced scattering coefficients and the absorption coefficients of in vivo exposed brain tissues in the range from visible to near-infrared wavelength (500-760 nm) based on diffuse reflectance spectroscopy using a digital RGB camera. In the proposed method, the multi-spectral reflectance images of in vivo exposed brain are reconstructed from the digital red, green, blue images using the Wiener estimation algorithm. The Monte Carlo simulation-based multiple regression analysis for the absorbance spectra is then used to specify the absorption and scattering parameters of brain tissue. In this analysis, the concentration of oxygenated hemoglobin and that of deoxygenated hemoglobin are estimated as the absorption parameters whereas the scattering amplitude a and the scattering power b in the expression of μ_s’=aλ^-b as the scattering parameters, respectively. The spectra of absorption and reduced scattering coefficients are reconstructed from the absorption and scattering parameters, and finally, the spectral images of absorption and reduced scattering coefficients are estimated. We performed simultaneous recordings of spectral diffuse reflectance images and of the electrophysiological signals for in vivo exposed rat brain during the cortical spreading depression evoked by the topical application of KCl. Changes in the total hemoglobin concentration and the tissue oxygen saturation imply the temporary change in cerebral blood flow during CSD. Change in the reduced scattering coefficient was observed before the profound increase in the total hemoglobin concentration, and its occurrence was synchronized with the negative dc shift of the local field potential.

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Izumi Nishidate, Tomohiro Ishizuka, Keiichiro Yoshida, Satoko Kawauchi, Shunichi Sato, and Manabu Sato "Visualization of hemodynamics and light scattering in exposed brain of rat using multispectral image reconstruction based on Wiener estimation method", Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360Z (14 July 2015); https://doi.org/10.1117/12.2183759

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KEYWORDS

Scattering

Absorption

Brain

Tissues

Reflectivity

Neuroimaging

In vivo imaging

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