Hybrid label-free multiphoton and optoacoustic microscopy (MPOM)

Dominik Soliman; George J. Tserevelakis; Murad Omar; Vasilis Ntziachristos

doi:10.1117/12.2183619

16 July 2015 Hybrid label-free multiphoton and optoacoustic microscopy (MPOM)

Dominik Soliman, George J. Tserevelakis, Murad Omar, Vasilis Ntziachristos

Proceedings Volume 9539, Opto-Acoustic Methods and Applications in Biophotonics II; 953908 (2015) https://doi.org/10.1117/12.2183619
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

Many biological applications require a simultaneous observation of different anatomical features. However, unless potentially harmful staining of the specimens is employed, individual microscopy techniques do generally not provide multi-contrast capabilities. We present a hybrid microscope integrating optoacoustic microscopy and multiphoton microscopy, including second-harmonic generation, into a single device. This combined multiphoton and optoacoustic microscope (MPOM) offers visualization of a broad range of structures by employing different contrast mechanisms and at the same time enables pure label-free imaging of biological systems. We investigate the relative performance of the two microscopy modalities and demonstrate their multi-contrast abilities through the label-free imaging of a zebrafish larva ex vivo, simultaneously visualizing muscles and pigments. This hybrid microscopy application bears great potential for developmental biology studies, enabling more comprehensive information to be obtained from biological specimens without the necessity of staining.

Citation Download Citation

Dominik Soliman, George J. Tserevelakis, Murad Omar, and Vasilis Ntziachristos "Hybrid label-free multiphoton and optoacoustic microscopy (MPOM)", Proc. SPIE 9539, Opto-Acoustic Methods and Applications in Biophotonics II, 953908 (16 July 2015); https://doi.org/10.1117/12.2183619

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KEYWORDS

Microscopy

Multiphoton microscopy

Microscopes

Second-harmonic generation

Mirrors

Visualization

Imaging systems

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