Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy

Roberto Savoia; Pasquale Memmolo; Francesco Merola; Lisa Miccio; Giuliana D'Ippolito; Angelo Fontana; Pietro Ferraro

doi:10.1117/12.2185016

13 July 2015 Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy

Roberto Savoia, Pasquale Memmolo, Francesco Merola, Lisa Miccio, Giuliana D'Ippolito, Angelo Fontana, Pietro Ferraro

Proceedings Volume 9540, Novel Biophotonics Techniques and Applications III; 95400J (2015) https://doi.org/10.1117/12.2185016
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

In this paper, we present a new approach for three-dimensional reconstruction and biovolume estimation of some species of diatoms. An optofluidic platform, composed by an optical tweezer and holographic modulus, is employed to retrieve several holograms at different angular positions, which are processed by the shape from silhouette algorithm to estimate the 3D shape of the cells.

Citation Download Citation

Roberto Savoia, Pasquale Memmolo, Francesco Merola, Lisa Miccio, Giuliana D'Ippolito, Angelo Fontana, and Pietro Ferraro "Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy", Proc. SPIE 9540, Novel Biophotonics Techniques and Applications III, 95400J (13 July 2015); https://doi.org/10.1117/12.2185016

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