A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice

Karina S. Litvinova; Shakil Ahmad; Keqing Wang; Ilya E. Rafailov; Sergei G. Sokolovski; Lin Zhang; Edik U. Rafailov; Asif Ahmed

doi:10.1117/12.2213026

29 February 2016 A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice

Karina S. Litvinova, Shakil Ahmad, Keqing Wang, Ilya E. Rafailov, Sergei G. Sokolovski, Lin Zhang, Edik U. Rafailov, Asif Ahmed

Author Affiliations +

Proceedings Volume 9689, Photonic Therapeutics and Diagnostics XII; 96893H (2016) https://doi.org/10.1117/12.2213026
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal conditions throughout the experiment and treatment.

Conference Presentation

Citation Download Citation

Karina S. Litvinova, Shakil Ahmad, Keqing Wang, Ilya E. Rafailov, Sergei G. Sokolovski, Lin Zhang, Edik U. Rafailov, and Asif Ahmed "A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice", Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96893H (29 February 2016); https://doi.org/10.1117/12.2213026

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available