Spatially offset raman spectroscopy for non-invasive assessment of fracture healing

Hao Ding; Guijin Lu; Christopher West; Gloria Gogola; James Kellam; Catherine Ambrose; Xiaohong Bi

doi:10.1117/12.2224493

29 February 2016 Spatially offset raman spectroscopy for non-invasive assessment of fracture healing

Hao Ding, Guijin Lu, Christopher West, Gloria Gogola, James Kellam, Catherine Ambrose, Xiaohong Bi

Proceedings Volume 9689, Photonic Therapeutics and Diagnostics XII; 96894M (2016) https://doi.org/10.1117/12.2224493
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Fracture non-unions and bone re-fracture are common challenges for post-fracture management. To achieve better prognosis and treatment evaluation, it is important to be able to assess the quality of callus over the time course of healing. This study evaluated the potential of spatially offset Raman spectroscopy for assessing the fracture healing process in situ. We investigated a rat model of fracture healing at two weeks and 4 weeks post fracture with a fractured femur and a contralateral control in each animal. Raman spectra were collected from the depilated thighs on both sides transcutaneously in situ with various source/detection offsets. Bone signals were recovered from SORS spectra, and then compared with those collected from bare bones. The relative intensity of mineral from fractured bone was markedly decreased compared to the control. The fractured bones demonstrated lower mineral and carbonate level and higher collagen content in the callus at the early time point. Compared to week 2, collagen mineralization and mineral carbonation increased at 4 weeks post fracture. Similarly, the material properties of callus determined by reference point indentation also increased in the 4-week group, indicating improved callus quality with time. The results from Raman analysis are in agreement with radiographic and material testing, indicating the potential of this technique in assessing fracture healing in vivo.

Conference Presentation

Citation Download Citation

Hao Ding, Guijin Lu, Christopher West, Gloria Gogola, James Kellam, Catherine Ambrose, and Xiaohong Bi "Spatially offset raman spectroscopy for non-invasive assessment of fracture healing", Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96894M (29 February 2016); https://doi.org/10.1117/12.2224493

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available