Targeted illumination and tracking using optical fiber probe for optogenetics application

Anant Shinde; Sandeep Menon Perinchery; Murukeshan Vadakke Matham

doi:10.1117/12.2211282

9 March 2016 Targeted illumination and tracking using optical fiber probe for optogenetics application

Anant Shinde, Sandeep Menon Perinchery, Murukeshan Vadakke Matham

Proceedings Volume 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation; 969028 (2016) https://doi.org/10.1117/12.2211282
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

There was a renewed interest, during the recent years, in the imaging and tracking of targeted cells or organelles for a variety of biomedical and lab-on a chip applications that include particles movement. However, nonspecific illumination during tracking can have adverse effects such as heating, reduced image contrast and photo bleaching. In fact, current available tracking and imaging systems are unable to selectively illuminate the particle being tracked. To fill this void, we have developed a fiber optics based probe system incorporating a spatial light modulator (SLM) and an imaging fiber bundle for selective illumination on the targeted particle. A GRIN lens is attached at the distal endface of the image fiber bundle for optimised illumination and collection. A tracking algorithm is developed in order to enable controlled illumination through SLM to target the illumination point or location in accordance with the particle movement and size variation. Further with this probe, particles can be illuminated with light pulses of controllable duty cycle and frequency. The proposed methodology and developed probe have good significance and expected to find potential applications areas such as optogenetics, cell signalling studies, and lab-on a chip systems.

Conference Presentation

Citation Download Citation

Anant Shinde, Sandeep Menon Perinchery, and Murukeshan Vadakke Matham "Targeted illumination and tracking using optical fiber probe for optogenetics application", Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 969028 (9 March 2016); https://doi.org/10.1117/12.2211282

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