Comparison of two photosensitizers in photodynamic therapy using light pulses in femtosecond regime: an animal study

Clóvis Grecco; Sebastião Pratavieira; Vanderlei Bagnato; Cristina Kurachi

doi:10.1117/12.2211140

1 March 2016 Comparison of two photosensitizers in photodynamic therapy using light pulses in femtosecond regime: an animal study

Clóvis Grecco, Sebastião Pratavieira, Vanderlei Bagnato, Cristina Kurachi

Author Affiliations +

Proceedings Volume 9694, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXV; 969417 (2016) https://doi.org/10.1117/12.2211140
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Photodynamic therapy is a therapeutic modality for cancer treatment based on the interaction of light with a sensitizer agent and molecular oxygen present into the target cells. The aim of this study is the evaluation of photodynamic therapy using pulsed light source in the femtosecond regime through necrosis induced in healthy rat liver. The induced necrosis profile with CW laser and pulsed laser were evaluated in animal model, which received Photodithazine (chlorine e6 derivative). The light sources used in these studies were a 660 nm CW diode laser and a Ti:Sapphire Regenerative Amplifier laser (1 kHz repetition rate and 100 fs pulse width) associated with an optical parametric amplifier (OPA) to convert to 660 nm. The results were compared with a previous study when was used a hematoporphyrin derivative (Photogem) as a sensitizer. The induced necrosis with Photogen was greater with pulsed laser (2.0 ± 0.2 mm) in comparison with CW laser (1.0 ± 0.2 mm), while in Photodithazine the induced necrosis with was greater with CW laser (2.9 ± 0.2 mm) comparing the pulsed laser (2.0 ± 0.2 mm). These results indicate dependence of PDT mechanisms with photosensitizer and the light regime applied.

Citation Download Citation

Clóvis Grecco, Sebastião Pratavieira, Vanderlei Bagnato, and Cristina Kurachi "Comparison of two photosensitizers in photodynamic therapy using light pulses in femtosecond regime: an animal study", Proc. SPIE 9694, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXV, 969417 (1 March 2016); https://doi.org/10.1117/12.2211140

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KEYWORDS

Pulsed laser operation

Photodynamic therapy

Continuous wave operation

Femtosecond phenomena

Light sources

Semiconductor lasers

Laser therapeutics

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