Low-power laser irradiation did not stimulate breast cancer cells following ionizing radiation

C. R. Silva; C. F. M. Camargo; F. V. Cabral; M. S. Ribeiro

doi:10.1117/12.2213506

8 March 2016 Low-power laser irradiation did not stimulate breast cancer cells following ionizing radiation

C. R. Silva, C. F. M. Camargo, F. V. Cabral, M. S. Ribeiro

Author Affiliations +

Proceedings Volume 9695, Mechanisms of Photobiomodulation Therapy XI; 96950H (2016) https://doi.org/10.1117/12.2213506
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Cancer has become a public health problem worldwide. Radiotherapy may be a treatment to a number of types of cancer, frequently using gamma-radiation with sources such as ¹³⁷Cs and ⁶⁰Co, with varying doses, dose rates, and exposure times to obtain a better as a stimulant for cell proliferation and tissue healing process. However, its effects on cancer cells are not yet well elucidated. The purpose of this work was to evaluate the effects of the LPL on breast cancer cultures after ionizing radiation. The breast cancer-MDA-MB-231 cells were gamma irradiated by a ⁶⁰Co source, with dose of 2.5 Gy. After 24h, cells were submitted to LPL irradiation using a red laser emitting at λ= 660 nm, with output power of 40 mW and exposure time of 30 s and 60 s. The plates were uniformly irradiated, with energy of 1.2 J and 2.4 J, respectively. Cell viability was analyzed using the exclusion method with trypan blue. Our results show that breast cancer cells submitted to LPL after ionizing radiation remained 95 % viable. No statistically significant differences were observed between laser and control untreated cells, (P > 0.05). These findings suggest that LPL did not influenced cancer cells viability.

Conference Presentation

Citation Download Citation

C. R. Silva, C. F. M. Camargo, F. V. Cabral, and M. S. Ribeiro "Low-power laser irradiation did not stimulate breast cancer cells following ionizing radiation", Proc. SPIE 9695, Mechanisms of Photobiomodulation Therapy XI, 96950H (8 March 2016); https://doi.org/10.1117/12.2213506

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