An integrated optical coherence microscopy imaging and optical stimulation system for optogenetic pacing in Drosophila melanogaster
(Conference Presentation)

Aneesh Alex; Airong Li; Jing Men; Jason Jerwick; Rudolph E. Tanzi; Chao Zhou; Zhan Zhang; Xianxu Zeng

doi:10.1117/12.2214671

26 April 2016 An integrated optical coherence microscopy imaging and optical stimulation system for optogenetic pacing in Drosophila melanogaster (Conference Presentation)

Aneesh Alex, Airong Li, Jing Men, Jason Jerwick, Rudolph E. Tanzi, Chao Zhou, Zhan Zhang, Xianxu Zeng

Author Affiliations +

Proceedings Volume 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX; 96971A (2016) https://doi.org/10.1117/12.2214671
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Electrical stimulation is the clinical standard for cardiac pacing. Although highly effective in controlling cardiac rhythm, the invasive nature, non-specificity to cardiac tissues and possible tissue damage limits its applications. Optogenetic pacing of the heart is a promising alternative, which is non-invasive and more specific, has high spatial and temporal precision, and avoids the shortcomings in electrical stimulation. Drosophila melanogaster, which is a powerful model organism with orthologs of nearly 75% of human disease genes, has not been studied for optogenetic pacing in the heart. Here, we developed a non-invasive integrated optical pacing and optical coherence microscopy (OCM) imaging system to control the heart rhythm of Drosophila at different developmental stages using light. The OCM system is capable of providing high imaging speed (130 frames/s) and ultrahigh imaging resolutions (1.5 μm and 3.9 μm for axial and transverse resolutions, respectively). A light-sensitive pacemaker was developed in Drosophila by specifically expressing the light-gated cation channel, channelrhodopsin-2 (ChR2) in transgenic Drosophila heart. We achieved non-invasive and specific optical control of the Drosophila heart rhythm throughout the fly’s life cycle (larva, pupa, and adult) by stimulating the heart with 475 nm pulsed laser light. Heart response to stimulation pulses was monitored non-invasively with OCM. This integrated non-invasive optogenetic control and in vivo imaging technique provides a novel platform for performing research studies in developmental cardiology.

Conference Presentation

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Aneesh Alex, Airong Li, Jing Men, Jason Jerwick, Rudolph E. Tanzi, Chao Zhou, Zhan Zhang, and Xianxu Zeng "An integrated optical coherence microscopy imaging and optical stimulation system for optogenetic pacing in Drosophila melanogaster (Conference Presentation)", Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 96971A (26 April 2016); https://doi.org/10.1117/12.2214671

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KEYWORDS

Imaging systems

Heart

Integrated optics

Optogenetics

Coherence imaging

Coherence (optics)

Microscopy

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