Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field

Andrea Curatolo; Martin Villiger; Dirk Lorenser; Philip Wijesinghe; Alexander Fritz; Brendan F. Kennedy; David D. Sampson

doi:10.1117/12.2214684

8 March 2016 Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field

Andrea Curatolo, Martin Villiger, Dirk Lorenser, Philip Wijesinghe, Alexander Fritz, Brendan F. Kennedy, David D. Sampson

Author Affiliations +

Proceedings Volume 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX; 96971Q (2016) https://doi.org/10.1117/12.2214684
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Visualizing stiffness within the local tissue environment at the cellular and sub-cellular level promises to provide insight into the genesis and progression of disease. In this paper, we propose ultrahigh-resolution optical coherence elastography, and demonstrate three-dimensional imaging of local axial strain of tissues undergoing compressive loading. The technique employs a dual-arm extended focus optical coherence microscope to measure tissue displacement under compression. The system uses a broad bandwidth supercontinuum source for ultrahigh axial resolution, Bessel beam illumination and Gaussian beam detection, maintaining sub-2 μm transverse resolution over nearly 100 μm depth of field, and spectral-domain detection allowing high displacement sensitivity. The system produces strain elastograms with a record resolution (x,y,z) of 2×2×15 μm. We benchmark the advances in terms of resolution and strain sensitivity by imaging a suitable inclusion phantom. We also demonstrate this performance on freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography system.

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Citation Download Citation

Andrea Curatolo, Martin Villiger, Dirk Lorenser, Philip Wijesinghe, Alexander Fritz, Brendan F. Kennedy, and David D. Sampson "Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field", Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 96971Q (8 March 2016); https://doi.org/10.1117/12.2214684

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