Three dimensional time lapse imaging of live cell mitochondria with photothermal optical lock-in optical coherence microscopy
(Conference Presentation)

Miguel Sison; Sabyasachi Chakrabortty; Jerome Extermann; Amir Nahas; Christophe Pache; Tanja Weil; Theo Lasser

doi:10.1117/12.2214761

26 April 2016 Three dimensional time lapse imaging of live cell mitochondria with photothermal optical lock-in optical coherence microscopy (Conference Presentation)

Miguel Sison, Sabyasachi Chakrabortty, Jerome Extermann, Amir Nahas, Christophe Pache, Tanja Weil, Theo Lasser

Author Affiliations +

Proceedings Volume 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX; 96971Y (2016) https://doi.org/10.1117/12.2214761
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The photothermal optical lock-in optical coherence microscope (poli-OCM) introduced molecular specificity to OCM imaging, which is conventionally, a label-free technique. Here we achieve three-dimensional live cell and mitochondria specific imaging using ~4nm protein-functionalized gold nanoparticles (AuNPs). These nanoparticles do not photobleach and we demonstrate they’re suitability for long-term time lapse imaging. We compare the accuracy of labelling with these AuNPs using classical fluorescence confocal imaging with a standard mitochondria specific marker. Furthermore, time lapse poli-OCM imaging every 5 minutes over 1.5 hours period was achieved, revealing the ability for three-dimensional monitoring of mitochondria dynamics.

Conference Presentation

Citation Download Citation

Miguel Sison, Sabyasachi Chakrabortty, Jerome Extermann, Amir Nahas, Christophe Pache, Tanja Weil, and Theo Lasser "Three dimensional time lapse imaging of live cell mitochondria with photothermal optical lock-in optical coherence microscopy (Conference Presentation)", Proc. SPIE 9697, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, 96971Y (26 April 2016); https://doi.org/10.1117/12.2214761

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