Medical diagnosis and remote sensing at fiber-tip: picosecond resolved FRET sensor

Nabarun Polley; Samir Kumar Pal

doi:10.1117/12.2207588

7 March 2016 Medical diagnosis and remote sensing at fiber-tip: picosecond resolved FRET sensor

Nabarun Polley, Samir Kumar Pal

Proceedings Volume 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI; 970211 (2016) https://doi.org/10.1117/12.2207588
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Förster Resonance Energy Transfer (FRET) strategy in popular in fiber-optic sensing. However, the steady state emission quenching of the donor is inadequate to conclude FRET. The resonance type energy transfer from one molecule (donor) to other (acceptor) should meet few key properties including donor to acceptor energy migration in non-radiative way. In the present study, we have coupled the evanescent field of an optical fiber to the covalently attached donor (dansyl) molecules at the fiber tip. By using picosecond resolved time correlated single photon counting (TCSPC) we have demonstrated that dansyl at the fiber tip transfers energy to a well known DNA-intercalating dye ethidium. Our ultrafast detection scheme selectively distinguishes the probe (dansyl) emission from the intrinsic emission of the fiber. We have also used the setup for the remote sensing of the dielectric constant (polarity) of an environment. We have finally implemented the detection mechanism to detect an industrial synthetic dye methylene blue (MB) in water.

Citation Download Citation

Nabarun Polley and Samir Kumar Pal "Medical diagnosis and remote sensing at fiber-tip: picosecond resolved FRET sensor", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 970211 (7 March 2016); https://doi.org/10.1117/12.2207588

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