Detection of advanced glycation end products (AGEs) on human skin by in vivo confocal Raman spectroscopy

A. A. Martin; L. Pereira; S. M. Ali; C. D. Pizzol; C. A. Tellez; P. P. Favero; L. Santos; V. V. da Silva; C. E. O. Praes

doi:10.1117/12.2213323

7 March 2016 Detection of advanced glycation end products (AGEs) on human skin by in vivo confocal Raman spectroscopy

A. A. Martin, L. Pereira, S. M. Ali, C. D. Pizzol, C. A. Tellez, P. P. Favero, L. Santos, V. V. da Silva, C. E. O. Praes

Author Affiliations +

Proceedings Volume 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry; 97040S (2016) https://doi.org/10.1117/12.2213323
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The aging process involves the reduction in the production of the major components of skin tissue. During intrinsic aging and photoaging processes, in dermis of human skin, fibroblasts become senescent and have decreased activity, which produce low levels of collagen. Moreover, there is accumulation of advanced glycation end products (AGEs). AGEs have incidence in the progression of age-related diseases, principally in diabetes mellitus and in Alzheimer's diseases. AGEs causes intracellular damage and/or apoptosis leading to an increase of the free radicals, generating a crosslink with skin proteins and oxidative stress. The aim of this study is to detect AGEs markers on human skin by in vivo Confocal Raman spectroscopy. Spectra were obtained by using a Rivers Diagnostic System, 785 nm laser excitation and a CCD detector from the skin surface down to 120 μm depth. We analyzed the confocal Raman spectra of the skin dermis of 30 women volunteers divided into 3 groups: 10 volunteers with diabetes mellitus type II, 65–80 years old (DEW); 10 young healthy women, 20–33 years old (HYW); and 10 elderly healthy women, 65–80 years old (HEW). Pentosidine and glucosepane were the principally identified AGEs in the hydroxyproline and proline Raman spectral region (1000–800 cm^–1), in the 1.260–1.320 cm^–1 region assignable to alpha-helical amide III modes, and in the Amide I region. Pentosidine and glucosepane calculated vibrational spectra were performed through Density Functional Theory using the B3LYP functional with 3-21G basis set. Difference between the Raman spectra of diabetic elderly women and healthy young women, and between healthy elderly women and healthy young women were also obtained with the purpose of identifying AGEs Raman bands markers. AGEs peaks and collagen changes have been identified and used to quantify the glycation process in human skin.

Conference Presentation

Citation Download Citation

A. A. Martin, L. Pereira, S. M. Ali, C. D. Pizzol, C. A. Tellez, P. P. Favero, L. Santos, V. V. da Silva, and C. E. O. Praes "Detection of advanced glycation end products (AGEs) on human skin by in vivo confocal Raman spectroscopy", Proc. SPIE 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, 97040S (7 March 2016); https://doi.org/10.1117/12.2213323

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available