Towards optical fibre based Raman spectroscopy for the detection of surgical site infection

Alex J. Thompson; Lukasz Koziej; Huw D. Williams; Daniel S. Elson; Guang-Zhong Yang

doi:10.1117/12.2212769

7 March 2016 Towards optical fibre based Raman spectroscopy for the detection of surgical site infection

Alex J. Thompson, Lukasz Koziej, Huw D. Williams, Daniel S. Elson, Guang-Zhong Yang

Proceedings Volume 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry; 970414 (2016) https://doi.org/10.1117/12.2212769
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Surgical site infections (SSIs) are common post-surgical complications that remain significant clinical problems, as they are associated with substantial mortality and morbidity. As such, there is significant interest in the development of minimally invasive techniques that permit early detection of SSIs. To this end, we are applying a compact, clinically deployable Raman spectrometer coupled to an optical fibre probe to the study of bacteria, with the long term goal of using Raman spectroscopy to detect infection in vivo. Our system comprises a 785 nm laser diode for excitation and a commercial (Ocean Optics, Inc.) Raman spectrometer for detection. Here we discuss the design, optimisation and validation of this system, and describe our first experiences interrogating bacterial cells (Escherichia coli) in vitro.

Citation Download Citation

Alex J. Thompson, Lukasz Koziej, Huw D. Williams, Daniel S. Elson, and Guang-Zhong Yang "Towards optical fibre based Raman spectroscopy for the detection of surgical site infection", Proc. SPIE 9704, Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry, 970414 (7 March 2016); https://doi.org/10.1117/12.2212769

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