Multicolor single-molecule imaging by spectral point-spread-function engineering (Conference Presentation)

Yoav Shechtman; Lucien E. Weiss; Adam S. Backer; William E. Moerner

doi:10.1117/12.2208982

26 May 2016 Multicolor single-molecule imaging by spectral point-spread-function engineering (Conference Presentation)

Yoav Shechtman, Lucien E. Weiss, Adam S. Backer, William E. Moerner

Proceedings Volume 9714, Single Molecule Spectroscopy and Superresolution Imaging IX; 97140L (2016) https://doi.org/10.1117/12.2208982
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We extend the information content of the microscope’s point-spread-function (PSF) by adding a new degree of freedom: spectral information. We demonstrate controllable encoding of a microscopic emitter’s spectral information (color) and 3D position in the shape of the microscope’s PSF. The design scheme works by exploiting the chromatic dispersion of an optical element placed in the optical path. By using numerical optimization we design a single physical pattern that yields different desired phase delay patterns for different wavelengths. To demonstrate the method’s applicability experimentally, we apply it to super-resolution imaging and to multiple particle tracking.

Conference Presentation

Citation Download Citation

Yoav Shechtman, Lucien E. Weiss, Adam S. Backer, and William E. Moerner "Multicolor single-molecule imaging by spectral point-spread-function engineering (Conference Presentation)", Proc. SPIE 9714, Single Molecule Spectroscopy and Superresolution Imaging IX, 97140L (26 May 2016); https://doi.org/10.1117/12.2208982

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