Confocal microscopy via multimode fibers: fluorescence bandwidth

Damien Loterie; Demetri Psaltis; Christophe Moser

doi:10.1117/12.2208017

15 March 2016 Confocal microscopy via multimode fibers: fluorescence bandwidth

Damien Loterie, Demetri Psaltis, Christophe Moser

Proceedings Volume 9717, Adaptive Optics and Wavefront Control for Biological Systems II; 97171C (2016) https://doi.org/10.1117/12.2208017
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We recently described a method for confocal reflection imaging through fibers, as a way to increase contrast when imaging unstained biological specimens. Using a transmission matrix, focused spots can be created at the distal end of a fiber. The backscattered field coming back from the sample can be filtered using optical correlation to obtain spatial selectivity in the detection. In this proceedings article, we briefly review the working principle of this method, and we discuss how the scheme could be adapted to confocal fluorescence imaging. In particular, we show simulations of the achievable detection bandwidth when using step-index multimode fibers as imaging devices.

Citation Download Citation

Damien Loterie, Demetri Psaltis, and Christophe Moser "Confocal microscopy via multimode fibers: fluorescence bandwidth", Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 97171C (15 March 2016); https://doi.org/10.1117/12.2208017

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