Computational illumination for real-time gigapixel phase microscopy
(Conference Presentation)

Laura Waller

doi:10.1117/12.2209006

27 April 2016 Computational illumination for real-time gigapixel phase microscopy (Conference Presentation)

Laura Waller

Proceedings Volume 9718, Quantitative Phase Imaging II; 971808 (2016) https://doi.org/10.1117/12.2209006
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

This talk will describe new source coding methods for rapid acquisition of high-resolution phase images across a very large field of view, using a modified commercial microscope with LED array illumination. Our methods are inspired by a hybrid combination of Fourier Ptychography and differential phase contrast imaging, combined with source multiplexing strategies for fast capture and significantly reduced data requirements. Such computational approaches to optical microscopy add significant new capabilities to commercial microscopes without much cost or hardware modification. We demonstrate our system on live samples in vivo, validating our results for unstained, label-free samples against other popular phase retrieval methods.

Conference Presentation

Citation Download Citation

Laura Waller "Computational illumination for real-time gigapixel phase microscopy (Conference Presentation)", Proc. SPIE 9718, Quantitative Phase Imaging II, 971808 (27 April 2016); https://doi.org/10.1117/12.2209006

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