One shot confocal microscopy based on wavelength/space conversion by use of multichannel spectrometer

Shuji Miyamoto; Eiji Hase; Ryuji Ichikawa; Takeo Mnamikawa; Takeshi Yasui; Hirotugu Yamamoto

doi:10.1117/12.2213814

7 March 2016 One shot confocal microscopy based on wavelength/space conversion by use of multichannel spectrometer

Shuji Miyamoto, Eiji Hase, Ryuji Ichikawa, Takeo Mnamikawa, Takeshi Yasui, Hirotugu Yamamoto

Proceedings Volume 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management; 97201C (2016) https://doi.org/10.1117/12.2213814
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Confocal laser microscope (CLM) has been widely used in the fields of the non-contact surface topography, biomedical imaging, and other applications, because of two-dimensional (2D) or three-dimensional (3D) imaging capability with the confocal effect and the stray light elimination. Although the conventional CLM has acquired the 2D image by mechanical scanning of the focused beam spot, further reduction of image acquisition time and the robustness to various disturbances are strongly required. To this end, it is essential to omit mechanical scanning for the image acquisition. In this article, we developed the scan-less, full-field CLM by combination of the line-focused CLM with the wavelength/1D-space conversion. This combination enables us to form the 2D focal array of a 2D rainbow beam on a sample and to encode the 2D image information of a sample on the 2D rainbow beam. The image-encoded 2D rainbow beam was decoded as a spectral line image by a multi-channel spectrometer equipped with a CMOS camera without the need for the mechanical scanning. The confocal full-field image was acquired during 0.23 ms with the lateral resolution of 26.3μm and 4.9μm for the horizontal and vertical directions, respectively, and the depth resolution of 34.9μm. We further applied this scan-less, full-field CLM for biomedical imaging of a sliced specimen and non-contact surface topography of an industry products. These demonstrations highlight a high potential of the proposed scan-less, full-field CLM.

Citation Download Citation

Shuji Miyamoto, Eiji Hase, Ryuji Ichikawa, Takeo Mnamikawa, Takeshi Yasui, and Hirotugu Yamamoto "One shot confocal microscopy based on wavelength/space conversion by use of multichannel spectrometer", Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 97201C (7 March 2016); https://doi.org/10.1117/12.2213814

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KEYWORDS

Confocal microscopy

Spectroscopy

Biomedical optics

Image acquisition

Objectives

3D image processing

Diffraction gratings

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