New confocal microscopy hyperspectral imager for NIR-emitting bioprobes: high spectral resolution for a wide spectral range
(Conference Presentation)

Stéphane Marcet; Antonio Benayas; Marta Quintanilla; Francesca Mangiarini; Marc Verhaegen; Fiorenzo Vetrone; Sébastien Blais-Ouellette

doi:10.1117/12.2213865

27 April 2016 New confocal microscopy hyperspectral imager for NIR-emitting bioprobes: high spectral resolution for a wide spectral range (Conference Presentation)

Stéphane Marcet, Antonio Benayas, Marta Quintanilla, Francesca Mangiarini, Marc Verhaegen, Fiorenzo Vetrone, Sébastien Blais-Ouellette

Author Affiliations +

Proceedings Volume 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII; 97210Q (2016) https://doi.org/10.1117/12.2213865
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Functional nanoscale materials are being extensively investigated for applications in biology and medicine and are ready to make significant contributions in the realization of exciting advancements in diverse areas of diagnostics and therapeutics. Aiming for more accurate, efficient, non-invasive and fast diagnostic tools, the use of near-infrared (NIR) light in the range of the 1st and 2nd biological window (NIR-I: 0.70-0.95 µm; NIR-II: 1.00-1.35 µm) provides deeper penetration depth into biological tissue, better image contrast, reduced phototoxicity and photobleaching. Consequently, NIR-based bioimaging became a quickly emerging field and manifold new NIR-emitting bioprobes have been reported. Since commercially available microscopes are not optimized for this kind of NPs, a new microscopy hyperspectral confocal imager has been developed to cover a broad spectral range (400 to 1700 nm) with high spectral resolution. The smallest spectral variation can be easily monitored thanks to the high spectral resolution (as low as 0.2 nm). This is possible thanks to a combination of an EMCCD and an InGaAs camera with a high resolution spectrometer. An extended number of NPs can be excited with a Ti:Sapphire laser, which provides tunable illumination within 690-1040 nm. Cells and tissues can be mapped in less than 100 ms, allowing in-vivo imaging. As a proof of concept, here we present the preliminary results of the spatial distribution of the fluorescence signal intensity from lanthanide doped nanoparticles incorporated into a system of biological interest. The temperature sub-mm gradient – analyzing the spectral features so gathered through an all-optical route is also thoroughly discussed.

Conference Presentation

Citation Download Citation

Stéphane Marcet, Antonio Benayas, Marta Quintanilla, Francesca Mangiarini, Marc Verhaegen, Fiorenzo Vetrone, and Sébastien Blais-Ouellette "New confocal microscopy hyperspectral imager for NIR-emitting bioprobes: high spectral resolution for a wide spectral range (Conference Presentation)", Proc. SPIE 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, 97210Q (27 April 2016); https://doi.org/10.1117/12.2213865

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Confocal microscopy

Spectral resolution

Hyperspectral imaging

Imaging systems

Nanoparticles

Biology

Diagnostics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years