Aptamer conjugated silver nanoparticles for the detection of interleukin 6

Andrea K. Locke; Nicole Norwood; Haley L. Marks; Monika Schechinger; George W. Jackson; Duncan Graham; Gerard L. Coté

doi:10.1117/12.2213896

22 April 2016 Aptamer conjugated silver nanoparticles for the detection of interleukin 6

Andrea K. Locke, Nicole Norwood, Haley L. Marks, Monika Schechinger, George W. Jackson, Duncan Graham, Gerard L. Coté

Proceedings Volume 9724, Plasmonics in Biology and Medicine XIII; 972412 (2016) https://doi.org/10.1117/12.2213896
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The controlled assembly of plasmonic nanoparticles by a molecular binding event has emerged as a simple yet sensitive methodology for protein detection. Metallic nanoparticles (NPs) coated with functionalized aptamers can be utilized as biosensors by monitoring changes in particle optical properties, such as the LSPR shift and enhancement of the SERS spectra, in the presence of a target protein. Herein we test this method using two modified aptamers selected for the protein biomarker interleukin 6, an indicator of the dengue fever virus and other diseases including certain types of cancers, diabetes, and even arthritis. IL6 works by inducing an immunological response within the body that can be either anti-inflammatory or pro-inflammatory. The results show that the average hydrodynamic diameter of the NPs as measured by Dynamic Light Scattering was ~42 nm. After conjugation of the aptamers, the peak absorbance of the AgNPs shifted from 404 to 408 nm indicating a surface modification of the NPs due to the presence of the aptamer. Lastly, preliminary results were obtained showing an increase in SERS intensity occurs when the IL-6 protein was introduced to the conjugate solution but the assay will still need to be optimized in order for it to be able to monitor varying concentration changes within and across the desired range.

Citation Download Citation

Andrea K. Locke, Nicole Norwood, Haley L. Marks, Monika Schechinger, George W. Jackson, Duncan Graham, and Gerard L. Coté "Aptamer conjugated silver nanoparticles for the detection of interleukin 6", Proc. SPIE 9724, Plasmonics in Biology and Medicine XIII, 972412 (22 April 2016); https://doi.org/10.1117/12.2213896

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available