Multiphoton imaging with high peak power VECSELs

Shamil Mirkhanov; Adrian H. Quarterman; Samuel Swift; Bavishna B. Praveen; Conor J. C. Smyth; Keith G. Wilcox

doi:10.1117/12.2210862

10 March 2016 Multiphoton imaging with high peak power VECSELs

Shamil Mirkhanov, Adrian H. Quarterman, Samuel Swift, Bavishna B. Praveen, Conor J. C. Smyth, Keith G. Wilcox

Proceedings Volume 9734, Vertical External Cavity Surface Emitting Lasers (VECSELs) VI; 973412 (2016) https://doi.org/10.1117/12.2210862
Event: SPIE LASE, 2016, San Francisco, California, United States

Abstract

Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.

Citation Download Citation

Shamil Mirkhanov, Adrian H. Quarterman, Samuel Swift, Bavishna B. Praveen, Conor J. C. Smyth, and Keith G. Wilcox "Multiphoton imaging with high peak power VECSELs", Proc. SPIE 9734, Vertical External Cavity Surface Emitting Lasers (VECSELs) VI, 973412 (10 March 2016); https://doi.org/10.1117/12.2210862

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