Cell sorting using efficient light shaping approaches

Andrew Bañas; Darwin Palima; Mark Villangca; Jesper Glückstad

doi:10.1117/12.2214165

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4 March 2016 Cell sorting using efficient light shaping approaches

Andrew Bañas, Darwin Palima, Mark Villangca, Jesper Glückstad

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Proceedings Volume 9764, Complex Light and Optical Forces X; 97640F (2016) https://doi.org/10.1117/12.2214165
Event: SPIE OPTO, 2016, San Francisco, California, United States

Abstract

Early detection of diseases can save lives. Hence, there is emphasis in sorting rare disease-indicating cells within small dilute quantities such as in the confines of lab-on-a-chip devices. In our work, we use optical forces to isolate red blood cells detected by machine vision. This approach is gentler, less invasive and more economical compared to conventional FACS systems. As cells are less responsive to plastic or glass beads commonly used in the optical manipulation literature, and since laser safety would be an issue in clinical use, we develop efficient approaches in utilizing lasers and light modulation devices. The Generalized Phase Contrast (GPC) method that can be used for efficiently illuminating spatial light modulators or creating well-defined contiguous optical traps is supplemented by diffractive techniques capable of integrating the available light and creating 2D or 3D beam distributions aimed at the positions of the detected cells. Furthermore, the beam shaping freedom provided by GPC can allow optimizations in the beam’s propagation and its interaction with the catapulted cells.

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Andrew Bañas, Darwin Palima, Mark Villangca, and Jesper Glückstad "Cell sorting using efficient light shaping approaches", Proc. SPIE 9764, Complex Light and Optical Forces X, 97640F (4 March 2016); https://doi.org/10.1117/12.2214165

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