Alternative soaking media for the FDA procedure in the detection of salmonella from tomatoes and spinach leaf using phage magnetoelastic biosensors

I-Hsuan Chen; Jiajia Hu; Fengen Wang; Shin Horikawa; James M. Barbaree; Bryan A. Chin

doi:10.1117/12.2227942

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17 May 2016 Alternative soaking media for the FDA procedure in the detection of salmonella from tomatoes and spinach leaf using phage magnetoelastic biosensors

I-Hsuan Chen, Jiajia Hu, Fengen Wang, Shin Horikawa, James M. Barbaree, Bryan A. Chin

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Proceedings Volume 9864, Sensing for Agriculture and Food Quality and Safety VIII; 986412 (2016) https://doi.org/10.1117/12.2227942
Event: SPIE Commercial + Scientific Sensing and Imaging, 2016, Baltimore, Maryland, United States

Abstract

Efforts were made to incorporate the phage Magnetoelastic (ME) biosensor in FDA's Spinach Soaking procedures according to FDA 2015 BAM method. Three soaking materials (Lactose broth, LB broth, and Peptone water) and various soaking times were investigated. Using merely 100 Salmonella cells spiked on the produce surfaces, the phage biosensors detected Salmonella within 5 hours when soaking tomatoes in LB broth as opposed to taking up to 24 hours. Salmonella was detected on spinach leaves within 7 hours. These phage ME biosensors provide a promising rapid detection platform using LB broth in FDA's soaking procedures while shortening the incubation period.

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I-Hsuan Chen, Jiajia Hu, Fengen Wang, Shin Horikawa, James M. Barbaree, and Bryan A. Chin "Alternative soaking media for the FDA procedure in the detection of salmonella from tomatoes and spinach leaf using phage magnetoelastic biosensors", Proc. SPIE 9864, Sensing for Agriculture and Food Quality and Safety VIII, 986412 (17 May 2016); https://doi.org/10.1117/12.2227942

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