Laser intensity modulated real time monitoring cell growth sensor for bioprocess applications

P. Kishore; P. Ravindra Babu; V. Rama Devi; T. Maunika; P. Soujanya; P. V. N. Kishore; D. Dinakar

doi:10.1117/12.2227592

29 April 2016 Laser intensity modulated real time monitoring cell growth sensor for bioprocess applications

P. Kishore, P. Ravindra Babu, V. Rama Devi, T. Maunika, P. Soujanya, P. V. N. Kishore, D. Dinakar

Proceedings Volume 9899, Optical Sensing and Detection IV; 98992A (2016) https://doi.org/10.1117/12.2227592
Event: SPIE Photonics Europe, 2016, Brussels, Belgium

Abstract

This article proposes an optical method for monitoring the growth of Escherichia coli in Luria Bertani medium and Saccharomyces cereviciae in YPD. Suitable light is selected which on interaction with the analyte under consideration, gets adsorption / scattered. Required electronic circuitry is designed to drive the laser source and to detect the intensity of light using Photo-detector. All these components are embedded and arranged in a proper way and monitored the growth of the microbs in real time. The sensors results are compared with standard techniques such as colorimeter, Nephelometer and hemocytometer. The experimental results are in good agreement with the existed techniques and well suitable for real time monitoring applications of the growth of the microbs.

Citation Download Citation

P. Kishore, P. Ravindra Babu, V. Rama Devi, T. Maunika, P. Soujanya, P. V. N. Kishore, and D. Dinakar "Laser intensity modulated real time monitoring cell growth sensor for bioprocess applications", Proc. SPIE 9899, Optical Sensing and Detection IV, 98992A (29 April 2016); https://doi.org/10.1117/12.2227592

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