Fluorescence lifetime image scanning microscopy

Ingo Gregor; Niels Rademacher; Max Tillmann; Matthias Patting; Jörg Enderlein; Felix Koberling

doi:10.1117/12.2625458

2 March 2022 Fluorescence lifetime image scanning microscopy

Ingo Gregor, Niels Rademacher, Max Tillmann, Matthias Patting, Jörg Enderlein, Felix Koberling

Proceedings Volume PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV; PC119670J (2022) https://doi.org/10.1117/12.2625458
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Simultaneous investigations of the spatial arrangement and relative composition of proteins are of crucial importance in live-cell imaging. It was recently shown that Image Scanning Microscopy (ISM) can double the confocal resolution. Additionally, fluorescence lifetime based imaging (FLIM) opens an additional dimension in multispecies identification and separation. We combine these two techniques using an array of single-photon detectors together with a multichannel TCSPC and time tagging system for time-resolved single photon detection via tens of channels. First results illustrate the spatial resolution improvement and the selection of suitable fluorescent dyes to allow for lifetime based multiplexing.

Citation Download Citation

Ingo Gregor, Niels Rademacher, Max Tillmann, Matthias Patting, Jörg Enderlein, and Felix Koberling "Fluorescence lifetime image scanning microscopy", Proc. SPIE PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV, PC119670J (2 March 2022); https://doi.org/10.1117/12.2625458

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