CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC12136 > Article
Presentation
30 May 2022 From phase imaging to CNN-based quantitative representation
Cédric P. Allier, Lionel Hervé, Chiara Paviolo, Ondrej Mandula, Olivier Cioni, William Pierré, Kiran Padmanabhan, Francesca Andriani, Sophie Morales
Author Affiliations +
Proceedings Volume PC12136, Unconventional Optical Imaging III; PC121360T (2022) https://doi.org/10.1117/12.2625595
Event: SPIE Photonics Europe, 2022, Strasbourg, France
Abstract
We present a CNN-based quantification pipeline for the imaging and analysis of adherent cell cultures. The imaging part features two CNNs dedicated to lens-free microscopy performing accelerated holographic reconstruction and phase unwrapping. The analysis part features CNNs estimating several cellular metrics. These CNNs maps phase image into 2D quantitative representations of cell positions and measurements. The outputs images are processed by a local maxima algorithm to obtain a list of cell measurements. Here, we discuss the performance and limitations of this CNN-based quantification pipeline. The advantage is the fast processing time, i.e. the analysis of ~10.000 cells in 10 seconds.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Cédric P. Allier, Lionel Hervé, Chiara Paviolo, Ondrej Mandula, Olivier Cioni, William Pierré, Kiran Padmanabhan, Francesca Andriani, and Sophie Morales "From phase imaging to CNN-based quantitative representation", Proc. SPIE PC12136, Unconventional Optical Imaging III, PC121360T (30 May 2022); https://doi.org/10.1117/12.2625595
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
;
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Phase imaging
Microscopy
3D image reconstruction
Holography
Live cell imaging
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top