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17 March 2023 A structured light generator device for super-resolution microscopy
Matteo Calvarese, Petra Paiè, Alessia Candeo, Gianmaria Calisesi, Francesco Ceccarelli, Gianluca Valentini, Roberto Osellame, Hai Gong, Mark Neil, Francesca Bragheri, Andrea Bassi
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Proceedings Volume PC12363, Multiscale Imaging and Spectroscopy IV; PC123630V (2023) https://doi.org/10.1117/12.2650364
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Structured Illumination microscopy is a super-resolution imaging technique based on sample fluorescence excitation with a spatially modulated light pattern. The pattern properties as well as the capability to shift it over sample determine the quality of the final images. At the current state of the art, pattern generation and translation require bulky and non-trivial optical setups. Here we propose an integrated optical device for the versatile generation and translation of the light pattern. This device can be used as light source for a standard microscope, upgrading it to a super-resolution system.
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Matteo Calvarese, Petra Paiè, Alessia Candeo, Gianmaria Calisesi, Francesco Ceccarelli, Gianluca Valentini, Roberto Osellame, Hai Gong, Mark Neil, Francesca Bragheri, and Andrea Bassi "A structured light generator device for super-resolution microscopy", Proc. SPIE PC12363, Multiscale Imaging and Spectroscopy IV, PC123630V (17 March 2023); https://doi.org/10.1117/12.2650364
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KEYWORDS
Structured light
Super resolution microscopy
Beam splitters
Image resolution
Spatial light modulators
Microscopy
Phase shifts
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