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Non-invasive imaging with high resolution deep within biological materials without the use of harmful ionizing radiation is of great interest in the field of medical imaging. Second harmonic generation is an excellent mechanism to circumvent this issue by providing outstanding contrast and optical sectioning. In general, these signals are weak and prone to scattering which introduce great challenges when imaging deep within turbid media. We will discuss recently demonstrated Epi-SHG holography, which can detect very weak backscattered SHG signals and enables the application of recently developed techniques which utilize the phase information to allow diffraction limited imaging within deep tissue.
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Gabe Murray, Jeff Field, Yusef Farah, Lang Wang, Maxine Xiu, Olivier Pinaud, Randy Bartels, "Towards deep imaging with EPI second harmonic generation holography," Proc. SPIE PC12363, Multiscale Imaging and Spectroscopy IV, PC123630W (17 March 2023); https://doi.org/10.1117/12.2650753