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6 March 2023 Photon counting with analog photodetection for high-signal-rate quantitative nonlinear microscopy
Geng Wang, Janet E. Sorrells, Rishyashring R. Iyer, Stephen A. Boppart, Haohua Tu
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Proceedings Volume PC12370, Design and Quality for Biomedical Technologies XVI; PC1237006 (2023) https://doi.org/10.1117/12.2648438
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
For high-signal-rate nonlinear microscopy using an analog PMT to detect multiple photons per excitation pulse, the conversion of analog output to photon counts is required to realize quantitative imaging. To enable this ability, we propose a method for photon counting with analog photodetection based on “imaging” of standard dye solutions and a mathematical model of Poisson photon statistics. Using this method, we not only enable the quantification across different types of PMTs from different setups, but also quantify the channel leakage for multichannel or multispectral imaging systems. This method paves the way for analog PMTs in high-signal-rate quantitative imaging of either label-free or labeled samples.
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Geng Wang, Janet E. Sorrells, Rishyashring R. Iyer, Stephen A. Boppart, and Haohua Tu "Photon counting with analog photodetection for high-signal-rate quantitative nonlinear microscopy", Proc. SPIE PC12370, Design and Quality for Biomedical Technologies XVI, PC1237006 (6 March 2023); https://doi.org/10.1117/12.2648438
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KEYWORDS
Photon counting
Analog electronics
Microscopy
Photodetectors
Imaging systems
Medical research
Neuroscience
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