CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC12383 > Article
Presentation
15 March 2023 Spectral imaging fluorescence flow cytometer for high-content analysis (Conference Presentation)
Yong Han, Jingjing Zhao, Zixi Chao, Zeheng Jiao, Attila Tárnok, Zheng You
Author Affiliations +
Proceedings Volume PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; PC1238304 (2023) https://doi.org/10.1117/12.2652682
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
In this work, we developed a new method for high throughput and high content spectral imaging flow cytometry based on structured linear spot array excitation. This method leverages equally spaced laser spots for illumination, scanning of a single cell with cell movement, and the cell image is reconstructed by splitting and assembling PMT signals. To demonstrate this method, we first built an imaging flow cytometer with dual-laser and five imaging channels (Bright-field, FITC, PE, PI, APC). More specially, due to the excellent scalability of this method, for the first time, we demonstrated a high-throughput hyperspectral imaging flow cytometer by integrating a high speed 32-channel spectrometer. This system obtains 32 spectral images of 1 μm resolution at the cell flowing speed of 5 m/s with the maximum throughput up to 5000 eps.
Conference Presentation
© (2023) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Yong Han, Jingjing Zhao, Zixi Chao, Zeheng Jiao, Attila Tárnok, and Zheng You "Spectral imaging fluorescence flow cytometer for high-content analysis (Conference Presentation)", Proc. SPIE PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, PC1238304 (15 March 2023); https://doi.org/10.1117/12.2652682
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Imaging spectroscopy
Luminescence
Signal detection
Image segmentation
Spectroscopy
Hyperspectral imaging
Image analysis
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top