Rapid block-face serial sectioning tomography for automated three-dimensional histological imaging (Conference Presentation)

Wentao Yu; Yan Zhang; Lei Kang; Claudia T. K. Lo; Terence T. W. Wong

doi:10.1117/12.2647791

15 March 2023 Rapid block-face serial sectioning tomography for automated three-dimensional histological imaging (Conference Presentation)

Wentao Yu, Yan Zhang, Lei Kang, Claudia T. K. Lo, Terence T. W. Wong

Author Affiliations +

Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC1238502 (2023) https://doi.org/10.1117/12.2647791
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

This paper proposes a fast whole-organ histological imaging method with real-time staining and mechanical sectioning. Time-consuming and laborious sample processing procedures are not needed. The imaged tissue block will be labeled along with the serial sectioning and optical scanning to improve the overall speed and the uniformity of staining. A super-resolution network (ESRGAN) and an optical-sectioning imaging technique (HiLo microscopy) have been applied to optimize the imaging speed and resolution. The proposed system can realize whole-organ histological imaging within hours to days, depending on the volume of the imaged sample.

Conference Presentation

Citation Download Citation

Wentao Yu, Yan Zhang, Lei Kang, Claudia T. K. Lo, and Terence T. W. Wong "Rapid block-face serial sectioning tomography for automated three-dimensional histological imaging (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC1238502 (15 March 2023); https://doi.org/10.1117/12.2647791

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available