Longitudinal drug response imaging of tumor spheroids using dynamic optical coherence tomography in conjunction with cell cultivation

Ibrahim G. Abd El-Sadek; Rion Morishita; Masato Iwatsuki; Atsuko Furukawa; Shuichi Makita; Pradipta Mukherjee; Satoshi Matsusaka; Yoshiaki Yasuno

doi:10.1117/12.3000669

13 March 2024 Longitudinal drug response imaging of tumor spheroids using dynamic optical coherence tomography in conjunction with cell cultivation

Ibrahim G. Abd El-Sadek, Rion Morishita, Masato Iwatsuki, Atsuko Furukawa, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, Yoshiaki Yasuno

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Proceedings Volume PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI; PC1284103 (2024) https://doi.org/10.1117/12.3000669
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We demonstrate longitudinal drug response imaging of tumor spheroids by integrating a spheroid cultivation chamber and the dynamic optical coherence tomography (DOCT) system. The cultivation chamber supports the spheroids with 5 % of CO2 and a temperature of 37 0C. In contrast to our previous cross-sectional time-course imaging method, this newly integrated system enabled longitudinal time-course imaging of a single sample, and hence enabled measuring large number of time-points of the same spheroid. It successfully revealed the temporal property of human breast cancer (MCF-7 cell-line) spheroid’s response to paclitaxel (PTX) and doxorubicin (DOX) with high-temporal-resolution.

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Ibrahim G. Abd El-Sadek, Rion Morishita, Masato Iwatsuki, Atsuko Furukawa, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, and Yoshiaki Yasuno "Longitudinal drug response imaging of tumor spheroids using dynamic optical coherence tomography in conjunction with cell cultivation", Proc. SPIE PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI, PC1284103 (13 March 2024); https://doi.org/10.1117/12.3000669

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