Large field-of-view 3D computational phase imaging using differential structured illumination microscopy (dSIM)

Alex C. Matlock; Zahid Yaqoob; Peter T. C. So

doi:10.1117/12.3001613

13 March 2024 Large field-of-view 3D computational phase imaging using differential structured illumination microscopy (dSIM)

Alex C. Matlock, Zahid Yaqoob, Peter T. C. So

Author Affiliations +

Proceedings Volume PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences; PC128570H (2024) https://doi.org/10.1117/12.3001613
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We developed differential structured illumination microscopy (dSIM) for efficiently imaging and reconstructing 3D biological samples at high-resolution over a large field-of-view. Using plane wave and grating-based structured illumination pairs in a differential illumination scheme, dSIM encodes scattering information from high-angle, traditionally nonlinear “darkfield” illuminations into linear intensity measurements enabling efficient 3D object reconstruction with linear inverse scattering models. This illumination scheme exceeds the 2X resolution limit enhancement of traditional phase-based SIM techniques. We reconstruct 3D objects with 4.5X better resolution than the coherent imaging bandwidth while maintaining an almost 1mm2 field-of-view. We illustrate this technique in simulation and experimentally on cell cultures and other living biological specimens.

Conference Presentation

Citation Download Citation

Alex C. Matlock, Zahid Yaqoob, and Peter T. C. So "Large field-of-view 3D computational phase imaging using differential structured illumination microscopy (dSIM)", Proc. SPIE PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences, PC128570H (13 March 2024); https://doi.org/10.1117/12.3001613

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available