Development of a combined soft x-ray and visible light microscope for observation of biological cells

Satoru Egawa; Kai Sakurai; Yoko Takeo; Kyota Yoshinaga; Shigeki Owada; Gota Yamaguchi; Shunya Yokomae; Hidekazu Mimura; Yutaka Yamagata; Makina Yabashi; Takashi Kimura; Mari Shimura

doi:10.1117/12.3028505

4 October 2024 Development of a combined soft x-ray and visible light microscope for observation of biological cells

Satoru Egawa, Kai Sakurai, Yoko Takeo, Kyota Yoshinaga, Shigeki Owada, Gota Yamaguchi, Shunya Yokomae, Hidekazu Mimura, Yutaka Yamagata, Makina Yabashi, Takashi Kimura, Mari Shimura

Author Affiliations +

Proceedings Volume PC13150, Advances in X-Ray/EUV Optics and Components XIX; PC1315001 (2024) https://doi.org/10.1117/12.3028505
Event: Optical Engineering + Applications, 2024, San Diego, California, United States

Abstract

We have been developing a transmission soft x-ray microscope utilizing Wolter mirrors at a soft x-ray beamline of SACLA. We upgraded the soft x-ray microscope to enable simultaneous visible light imaging and soft x-ray imaging. To achieve this, we divided the annular apertures of the condenser and objective Wolter mirrors into two sections, allowing for soft x-ray imaging with one part and visible light imaging with the other. Our microscopy allows imaging cells with fluorescent labels by visible light while observing them with water window soft x-rays, which is useful for studying living cells.

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Satoru Egawa, Kai Sakurai, Yoko Takeo, Kyota Yoshinaga, Shigeki Owada, Gota Yamaguchi, Shunya Yokomae, Hidekazu Mimura, Yutaka Yamagata, Makina Yabashi, Takashi Kimura, and Mari Shimura "Development of a combined soft x-ray and visible light microscope for observation of biological cells", Proc. SPIE PC13150, Advances in X-Ray/EUV Optics and Components XIX, PC1315001 (4 October 2024); https://doi.org/10.1117/12.3028505

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