Light scattering and morphology of the lymphocyte as applied to flow cytometry for distinguishing healthy and infected individuals

Gennady I. Ruban; Vladimir V. Berdnik; Dmitry V. Marinitch; Natalia V. Goncharova; Valery A. Loiko

doi:10.1117/1.3503404

1 September 2010 Light scattering and morphology of the lymphocyte as applied to flow cytometry for distinguishing healthy and infected individuals

Gennady I. Ruban, Vladimir V. Berdnik, Dmitry V. Marinitch, Natalia V. Goncharova, Valery A. Loiko

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Journal of Biomedical Optics, Vol. 15, Issue 5, 057008 (September 2010). https://doi.org/10.1117/1.3503404

Abstract

A simple optical model of single lymphocytes with smooth and nonsmooth surfaces has been developed for healthy and infected individuals. The model can be used for rapid (in the real-time scale) solution of the inverse light-scattering problem on the basis of optical data measured by label-free flow cytometry. Light scattering patterns have been calculated for the model developed. It has been shown that the smooth and nonsmooth cells can be resolved using the intensities of the sideward- and backward-scattered light. We have found by calculations and validated by the flow cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flow cytometry detection of individuals infected with viruses of hepatitises B or C and some others viruses are presented.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Gennady I. Ruban, Vladimir V. Berdnik, Dmitry V. Marinitch, Natalia V. Goncharova, and Valery A. Loiko "Light scattering and morphology of the lymphocyte as applied to flow cytometry for distinguishing healthy and infected individuals," Journal of Biomedical Optics 15(5), 057008 (1 September 2010). https://doi.org/10.1117/1.3503404

Published: 1 September 2010

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