Evaluation of transdermal delivery of nanoemulsions in ex vivo porcine skin using two-photon microscopy and confocal laser-scanning microscopy

Sanghoon Choi; Jin Woong Kim; Yong Joong Lee; Thomas Delmas; Changhwan Kim; Soyeun Park; Ho Lee

doi:10.1117/1.JBO.19.10.106006

9 October 2014 Evaluation of transdermal delivery of nanoemulsions in ex vivo porcine skin using two-photon microscopy and confocal laser-scanning microscopy

Sanghoon Choi, Jin Woong Kim, Yong Joong Lee, Thomas Delmas, Changhwan Kim, Soyeun Park, Ho Lee

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Journal of Biomedical Optics, Vol. 19, Issue 10, 106006 (October 2014). https://doi.org/10.1117/1.JBO.19.10.106006

Abstract

This study experimentally evaluates the self-targeting ability of asiaticoside-loaded nanoemulsions compared with nontargeted nanoemulsions in ex vivo experiments with porcine skin samples. Homebuilt two-photon and confocal laser-scanning microscopes were employed to noninvasively examine the transdermal delivery of two distinct nanoemulsions. Prior to the application of nanoemulsions, we noninvasively observed the morphology of porcine skin using two-photon microscopy. We have successfully visualized the distributions of the targeted and nontargeted nanoemulsions absorbed into the porcine skin samples. Asiaticoside-loaded nanoemulsions showed an improved ex vivo transdermal delivery through the stratum corneum compared with nonloaded nanoemulsions. As a secondary measure, nanoemulsions-applied samples were sliced in the depth direction with a surgical knife in order to obtain the complete depth-direction distribution profile of Nile red fluorescence. XZ images demonstrated that asiaticoside-loaded nanoemulsion penetrated deeper into the skin compared with nontargeted nanoemulsions. The basal layer boundary is clearly visible in the case of the asiaticoside-loaded skin sample. These results reaffirm the feasibility of using self-targeting ligands to improve permeation through the skin barrier for cosmetics and topical drug applications.

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Sanghoon Choi, Jin Woong Kim, Yong Joong Lee, Thomas Delmas, Changhwan Kim, Soyeun Park, and Ho Lee "Evaluation of transdermal delivery of nanoemulsions in ex vivo porcine skin using two-photon microscopy and confocal laser-scanning microscopy," Journal of Biomedical Optics 19(10), 106006 (9 October 2014). https://doi.org/10.1117/1.JBO.19.10.106006

Published: 9 October 2014

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