CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 19 > Issue 7 > Article
Open Access
18 July 2014 In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate
Veronika Huntosova, Sandrine Gay, Patrycja M. Nowak-Sliwinska, Senthil K. Rajendran, Matthieu Zellweger, Hubert van den Bergh, Georges Wagnières
Author Affiliations +
Journal of Biomedical Optics, Vol. 19, Issue 7, 077004 (July 2014). https://doi.org/10.1117/1.JBO.19.7.077004
Abstract
Measuring tissue oxygenation in vivo is of interest in fundamental biological as well as medical applications. One minimally invasive approach to assess the oxygen partial pressure in tissue (pO2) is to measure the oxygen-dependent luminescence lifetime of molecular probes. The relation between tissue pO2 and the probes’ luminescence lifetime is governed by the Stern-Volmer equation. Unfortunately, virtually all oxygen-sensitive probes based on this principle induce some degree of phototoxicity. For that reason, we studied the oxygen sensitivity and phototoxicity of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate [Ru(Phen)] using a dedicated optical fiber–based, time-resolved spectrometer in the chicken embryo chorioallantoic membrane. We demonstrated that, after intravenous injection, Ru(Phen)’s luminescence lifetime presents an easily detectable pO2 dependence at a low drug dose (1  mg/kg) and low fluence (120  mJ/cm2 at 470 nm). The phototoxic threshold was found to be at 10  J/cm2 with the same wavelength and drug dose, i.e., about two orders of magnitude larger than the fluence necessary to perform a pO2 measurement. Finally, an illustrative application of this pO2 measurement approach in a hypoxic tumor environment is presented.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2014/$25.00 © 2014 SPIE
Citation Download Citation
Veronika Huntosova, Sandrine Gay, Patrycja M. Nowak-Sliwinska, Senthil K. Rajendran, Matthieu Zellweger, Hubert van den Bergh, and Georges Wagnières "In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate," Journal of Biomedical Optics 19(7), 077004 (18 July 2014). https://doi.org/10.1117/1.JBO.19.7.077004
Published: 18 July 2014
JOURNAL ARTICLE
 12 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 22 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Luminescence
Content addressable memory
Oxygen
In vivo imaging
Blood
Tumors
Tissues
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top