En-face optical coherence tomography/fluorescence endomicroscopy for minimally invasive imaging using a robotic scanner

Manuel J. Marques; Michael R. Hughes; Khushi Vyas; Andrew Thrapp; Haojie Zhang; Adrian Bradu; Grigory Gelikonov; Petros Giataganas; Christopher J. Payne; Guang-Zhong Yang; Adrian Podoleanu

doi:10.1117/1.JBO.24.6.066006

20 June 2019 En-face optical coherence tomography/fluorescence endomicroscopy for minimally invasive imaging using a robotic scanner

Manuel J. Marques, Michael R. Hughes, Khushi Vyas, Andrew Thrapp, Haojie Zhang, Adrian Bradu, Grigory Gelikonov, Petros Giataganas, Christopher J. Payne, Guang-Zhong Yang, Adrian Podoleanu

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Journal of Biomedical Optics, Vol. 24, Issue 6, 066006 (June 2019). https://doi.org/10.1117/1.JBO.24.6.066006

Abstract

We report a compact rigid instrument capable of delivering en-face optical coherence tomography (OCT) images alongside (epi)-fluorescence endomicroscopy (FEM) images by means of a robotic scanning device. Two working imaging channels are included: one for a one-dimensional scanning, forward-viewing OCT probe and another for a fiber bundle used for the FEM system. The robotic scanning system provides the second axis of scanning for the OCT channel while allowing the field of view (FoV) of the FEM channel to be increased by mosaicking. The OCT channel has resolutions of 25 / 60 μm (axial/lateral) and can provide en-face images with an FoV of 1.6 × 2.7 mm². The FEM channel has a lateral resolution of better than 8 μm and can generate an FoV of 0.53 × 3.25 mm² through mosaicking. The reproducibility of the scanning was determined using phantoms to be better than the lateral resolution of the OCT channel. Combined OCT and FEM imaging were validated with ex-vivo ovine and porcine tissues, with the instrument mounted on an arm to ensure constant contact of the probe with the tissue. The OCT imaging system alone was validated for in-vivo human dermal imaging with the handheld instrument. In both cases, the instrument was capable of resolving fine features such as the sweat glands in human dermal tissue and the alveoli in porcine lung tissue.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Manuel J. Marques, Michael R. Hughes, Khushi Vyas, Andrew Thrapp, Haojie Zhang, Adrian Bradu, Grigory Gelikonov, Petros Giataganas, Christopher J. Payne, Guang-Zhong Yang, and Adrian Podoleanu "En-face optical coherence tomography/fluorescence endomicroscopy for minimally invasive imaging using a robotic scanner," Journal of Biomedical Optics 24(6), 066006 (20 June 2019). https://doi.org/10.1117/1.JBO.24.6.066006

Received: 11 February 2019; Accepted: 30 May 2019; Published: 20 June 2019

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