PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
SPIE publishes accepted journal articles as soon as they are approved for publication. Journal issues are considered In Progress until all articles for an issue have been published. Articles published ahead of the completed issue are fully citable.
Imaging deep structures with optical coherence tomography (OCT) is difficult in highly scattering biological tissue, such as the sclera. There is a need to visualize the suprachoroidal space and choroid through the sclera to study suprachoroidal drug delivery.
Aim
We aim to develop optical methods to image through the highly scattering sclera with a custom-built OCT system to visualize the suprachoroidal space and drug delivery within.
Approach
We developed a custom handheld OCT scanner to image the anterior segment and suprachoroidal space in ex vivo eye models. Tartrazine (Yellow 5) solution, which has been shown to optically clear biological tissue in the visible regime, was tested as a clearing agent to optimize near infrared OCT imaging through the sclera.
Results
Tartrazine dramatically increased OCT signal return from the deeper sclera and choroid and thus enabled visualization of the suprachoroidal drug delivery after transscleral injection.
Conclusions
We demonstrated successful optical clearing of the thick, porcine sclera with a compact handheld OCT system to image the suprachoroidal space. We believe there is broader potential to use optical clearing with handheld OCT for a variety of previously inaccessible, highly scattering tissue samples.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
For therapeutic approaches for upper tract urothelial carcinomas, the absorption μa and reduced scattering μs′ coefficients of these tissues are essential parameters to quantitatively evaluate the distribution of light treatment effects.
Aim
The μa and μs′ spectra of the human ureter, fatty tissue, ureteral and renal pelvic carcinomas, and porcine ureter and fatty tissue are measured over 400 to 700 nm to evaluate projected light penetration depths δ.
Approach
The optical properties were determined with a double integrating sphere optical system and inverse Monte Carlo methods. δ was calculated and compared between normal and cancerous human tissues as well as between normal human and porcine tissues.
Results
μa and μs′ spectra of each tissue were determined. The δ of the normal human ureter was less than those of the ureteral and renal pelvic carcinomas, whereas that of the porcine ureter was similar to that of the human ureter over 400 to 600 nm and ∼0.2 times smaller above 600 nm.
Conclusion
Optical properties of human and porcine upper urinary tracts provide insights into light distributions and the validity of ex vivo porcine models in preclinical evaluations of laser treatments.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.