Artificial testing targets with controllable blur for adaptive optics microscopes

Masayuki Hattori; Yosuke Tamada; Takashi Murata; Shin Oya; Mitsuyasu Hasebe; Yutaka Hayano; Yasuhiro Kamei

doi:10.1117/1.OE.56.8.080502

28 August 2017 Artificial testing targets with controllable blur for adaptive optics microscopes

Masayuki Hattori, Yosuke Tamada, Takashi Murata, Shin Oya, Mitsuyasu Hasebe, Yutaka Hayano, Yasuhiro Kamei

Author Affiliations +

Optical Engineering, Vol. 56, Issue 8, 080502 (August 2017). https://doi.org/10.1117/1.OE.56.8.080502

Abstract

This letter proposes a method of configuring a testing target to evaluate the performance of adaptive optics microscopes. In this method, a testing slide with fluorescent beads is used to simultaneously determine the point spread function and the field of view. The point spread function is reproduced to simulate actual biological samples by etching a microstructure on the cover glass. The fabrication process is simplified to facilitate an onsite preparation. The artificial tissue consists of solid materials and silicone oil and is stable for use in repetitive experiments.

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Masayuki Hattori, Yosuke Tamada, Takashi Murata, Shin Oya, Mitsuyasu Hasebe, Yutaka Hayano, and Yasuhiro Kamei "Artificial testing targets with controllable blur for adaptive optics microscopes," Optical Engineering 56(8), 080502 (28 August 2017). https://doi.org/10.1117/1.OE.56.8.080502

Received: 28 May 2017; Accepted: 3 August 2017; Published: 28 August 2017

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