Proceedings Article | 14 August 2019
Proc. SPIE. 11070, 17th International Photodynamic Association World Congress
KEYWORDS: Visible radiation, Light sources, Photodynamic therapy, Teeth, Oxygen, Toxicity, Spectrophotometry, In vitro testing, Absorption, Bacteria
This study evaluated Curcuma longa, Citrus lemon, Hamamelis virginiana and Hypericum perforatum available on the market as photosensitizers (FS) in Antimicrobial Photodynamic Therapy (aPDT). Each FS were analyzed in a spectrophotometer between 350 and 750 nm, to determine the ideal light source. Once the absorption bands were determined, 3 light sources were selected to determine the concentration of use, the compounds were verified at different concentrations on bovine dentin samples to evaluate the risk of staining. Once the concentration was determined, FS were evaluated for cytotoxicity on fibroblast culture. Each compound was then irradiated with each light source and evaluated for indirect reactive oxygen species (ROS) production. The bacterial reduction was tested in culture of E. faecalis in planktonic form and in biofilm using energy of 10 J and Energy Density of 26 J/cm2. The tested compounds had light absorption in three bands of the visible spectrum: violet (405 nm), blue (470 nm) and red (660 nm). At 1:6 concentration, none of the compounds promoted tooth staining, as none of the compounds showed significant toxicity in cells or bacterial suspension. In addition, when the compounds were irradiated at each wavelength, significant ROS production was observed. When the aPDT was performed on the planktonic and biofilm bacteria, significant microbial reduction was observed for both cases, reaching up to 5Logs of reduction. In conclusion, extracts of Curcuma longa, Citrus lemon, Hamamelis virginiana and Hypericum perforatum, showed potential for use in aPDT as photosensitizing agents.
